DAYA HAMBAT EKSTRAK DAUN DEWANDARU (Eugenia uniflora L.) TERHADAP AKTIVITAS GLUTATION S-TRANSFERASE PARU TIKUS SECARA IN VITRO

Abstract

Objective: In some tumor cases, the Glutathione S-Transferase (GST) have been known increased. It causes anticancer drugs would be metabolized faster. Dewandaru leaves extracts were shown inhibitory effect on GST rat liver activity.This research aims are to determine the inhibitory potency of chloroform, ethyl acetate, and ethanol extract of Dewandaru leaves on GST activity of rat pulmo by in vitro using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate. Methods: The research was performed by using cytosolic fraction of rat pulmo which is prepared by centrifugation. Chloroform, ethyl acetate, and ethanol extracts of Dewandaru leaves were obtained by maceration. The protein content of cytosolic fraction was measured by Lowry method using bovine serum albumin (BSA) as standard. The GST activity was known from the conjugated product between Glutathione (GSH) and the substrate. It was measured by spectrophotometer. IC50 of each extract was determined from the plots of remaining activities versus the (varied) concentration of extract at a fixed concentration of substrate. Results: The research results showed all extracts have inhibitory effect on GST activity. IC50 values of chloroform, ethyl acetate, and ethanol extract using CDNB as a substrate are 77.964; 299.305; 160.014 µg/ml respectively. Conclusions: Dewandaru leaves extracts were shown inhibitory effect on GST rat pulmo activity by in vitro using CDNB as substrate.