CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.) AGAINTS WiDr CELL

Abstract

Ethanol extract of the leaves salaplant (CynometraramifloraLinn.) have cytotoxic activity against WiDr cells with IC50 of 6.37 μg/mL. This study aims to determine the cytotoxic activity of polar, semipolar, and nonpolar fractions of ethanol extract of leaves sala plant (CynometraramifloraLinn.) against WiDr cells. Fractionation was performed on Vacuum Liquid Chromatography method with silica G 60 as stationary phase and n-hexane: ethylacetate(8:2; 7.5:2.5; 7:3,6:4 and 3:7) and ethanol as mobile phase. Qualitative screening of major compounds used TLC method with spray reagent. Cytotoxicity determination performedin vitro model cell lines with MTTreagent.The result showed that IC50 of polar, semi polar and non polar fractions were 231.95 μg/mL and cannot determined. Doxorubicin was used as positive control and obtained IC50 of 1.721 μg/mL.