MICROBIOLOGYActivities of The Combined Extracts of Tempuyung (Sonchus arvensis) and Black Cumin (Nigella sativa) Against Xanthine Oxidase Inhibition on Hyperuricemic Mice

Abstract

The combination extracts of tempuyung (Sonchus arvensis) and black cumin (Nigella sativa) can decrease uric acid levels on the previous result research. However, the mechanism of decreasing uric acid levels was unknown certainly. This study aimed to determine the inhibitory effect of xanthine oxidase by the combination extracts of black cumin and tempuyung on hyperuricemic mice. Hyperuricemic mice were induced by 250 mg/kgBW potassium oxonate was given one hour before treatment. The mice were divided into 3 groups, group I was given 10 mg/kgBW allopurinol (positive control), group II was given 0.5 mL/20gBW aquadest (negative control) and group III was given the combination extracts of black cumin-tempuyung with dose 200 mg/kgBW during 4 days administration. The supernatant of liver was taken and measured of xanthine oxidase levels by spectrophotometer UV at lamda 290 nm. The data of xanthine oxidase activity were analyzed by Kruskal-Wallis and Mann-Whitney method. Xanthine oxidase activity of the combination extracts of blackcumin-tempuyung was 4.54±0.9 U/mg, very significantly than control negative was 8.00±0.22 U/mg (p<0.05). Inhibition xanthine oxidase by the combination extracts of black cumin-tempuyung was 43.26±11.29%, lower than allopurinol was 90.19±0.36%.