Pengaruh Pemberian Campuran Bee Pollen, Rimpang Kencur, Kunyit dan Biji Pinang Terhadap Penurunan Kadar Malondialdehida (MDA) pada Tikus Wistar Pasca Paparan Streptozotocin

Abstract

This purpose of this research was to determine the effect of a mixture of bee po llen, rhizome of Kaempf eria Galanga po wder, Curcuma longa powder, betel nut, and cinnamomum po wder to a decrease in MDA levels in wistar rats after Streptozotocin-indu ced. Streptozo tocin work s by da maging pancreatic β cell. Mecha nism of pancreatic β cell damage du e to alk ylation of DNA methylation inhibits the secretion of insulin, causing the levels of MDA ride. Mice w ith the age of 11th months, divided into 6 groups rando mly. Group A was a negat ive c ontrol group, whic h was made w ithout the a dministration of STZ a nd mixture of extract orally. Group B was the positive contro l group, the rats were given STZ and without a mixture of extract. Group C to E was treatment grou p (mixture of bee bee pollen, rhizome of Kaempferia Galanga po wder, Curcuma longa powder, betel nut, and cinna momum with a ra ting dose of 200 mg/kgBB , 250 mg/kgBB , dan 300 mg/kgBB dur ing initia tion). Dosage, f requency and mode of ad ministration of STZ sa me as the po sitive control group. While the grou p F was a group of rats that were given STZ, metrofin and without a mixture of extract. The Sa mples of plasma was measured malondialdehyde level. Malondialdehyde was measured by TBARS method e, which measures the concentration of thiobarbituric Acid Reactive Sub stance. The absorbance was measured with a wavelength of 532 nm. A mixture of extract was ab le to provide reduced levels of MDA in rats with Streptozo tocin- induced, but from each treatment group there was no significant difference.