| dc.identifier.citation | Amaliah, A. R., 2014, Pengaruh Infus Daun Chitolod (Laurentia longiflora) Terhadap Histopatologi Mata Tikus Wistar Katarak yang Diinduksi Methyl Nitroso Urea., Universitas Katolik Widya Mandala, Surabaya. American Cancer Society, 2015, Breast Cancer Facts & Figures 2015-2016, Breast Cancer Facts & Figures 2015-2016. American Cancer Society, 2016, Cancer Facts & Figures 2016, Cancer Facts & Figures 2016, 1–9. Anderson, B. O., 2014, Breast Cancer, Cancer Research UK. England and Wales, Scotland and the Isle of Man. Bansal T., Jaggi M., Khar R.K. and Talegaonkar S., 2009, Emerging significance of flavonoids as P-glycoprotein inhibitors in cancer chemotherapy, Journal of Pharmacy and Pharmaceutical Sciences, 12 (1), 46–78. BPOM RI, 2010, Acuan Sediaan Herbl, 1st ed., Badan Pengawas Obat dan Makanan Republik Indonesia, Jakarta. Djajanegara I. and Wahyudi P., 2009, Pemakaian sel HeLa dalam uji sitotoksisitas fraksi kloroform dan etanol ekstrak daun Annona squamosa, Jurnal Ilmu Kefarmasian Indonesia, 7 (1), 7–11. Hapsari A., Asti D., Hidayati R., Kumalla N. and Suhendi A., 2016, The Potency of Chitolod (Isotoma longiflora (L) Presl.) Herb Extract as a Cure for Cervical Cancer: an in Vitro Study of Hela Cells, (L), 109–114. Harborne J.B., 1987, Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan, Penerbit ITB, Bandung. Hariana A., 2008, Tumbuhan Obat dan Khasiatnya, Cetakan ke-5, Penebar Swadaya, Jakarta. Haryoto, Irjayanti A.N., Suhendi A., Muhtadi and Sujono T.A., 2015, Cytotoxic Activity of Polar, Semipolar, and Nonpolar Fraction of Ethanol Extract of Sala Plants Leaves (Cynometra ramiflora Linn.) Againts WiDr Cell, Dalam ICB Pharma II “Current Breakthrough in Pharmacy Materials and Analyses,” Faculty of Pharmacy, Universitas Muhammadiyah Surakarta Surakarta, Central Java, Indonesia, pp. 60–64. Ismailova M., 2008, Uji Aktivitas Antibakteri Ekstrak Air Daun Chitolod (Laurentia longiflora (L.) Peterm.) Terhadap Bakteri yang Diisolasi dari Pasien Penderita Konjungtivitis,. Institut Teknologi Bandung. Ismiyati N., Mardiyaningsing A. and Trilestari, 2015, Aktivitas Sitotoksik Ekstrak Eetanolik dan fraksi dari Ekstrak Etanolik Daun Pandan (Pandanus amaryllifolius ROXB) Terhadap Sel Kanker Payudara MCF-7, The 2nd University Research Coloquium 2015, 343–348. Kementerian Kesehatan RI, 2015, Stop Kanker, Infodatin Kanker, Jakarta. Koller E., 2009, Javanese Medicinal Plants used in Rural Communities,. Wien University. Kurnijasanti R., Hamid I.S. and Rahmawati K., 2008, Efek sitotoksik in vitro dari ekstrak buah mahkota dewa (Phaleria macrocarpa) terhadap kultur sel kanker mieloma, J. Penelit. Med. Eksakta, 7 (1), 48–54. Magfiroh L., 2015, Uji Sitotoksisitas Fraksi-Fraksi Hasil Pemisahan Crude Extract Daun Chitolod (Isotoma longiflora L.) Terhadap Cell Line Kanker Kolon WiDr,. Universitas Islam Negeri Sunan Kalijaga. Marliana S.D., Suryanti V. and Suyono, 2005, Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol, Biofarmasi, 3 (1), 26–31. Maulida R. and Guntarti A., 2015, Pengaruh Ukuran Partikel Beras Hitam (Oryza Sativa L.) Terhadap Rendemen Ekstrak dan Kandungan Total Antosianin, Pharmaciana, 5, 9–16. McMurry and Fay, 2004, Chemistry, 4th Edition., Pearson Education International., Belmont. Prayong P., Barusrux S. and Weerapreeyakul N., 2008, Cytotoxic activity screening of some indigenous Thai plants, Fitoterapia, 79 (7-8), 598–601. Purwaningsih E., Widayanti E. and Suciati Y., 2014, Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells, 33 (2), 75–82. Putri H., 2013, Protokol Uji Sitotoksik Metode MTT, CCRC, Universitas Gadjah Mada, Yogyakarta. Ren W., Qiao Z., Wang H., Zhu L. and Zhang L., 2003, Flavonoids: Promising Anticancer Agents, Medicinal Research Reviews, 23 (4). Rothan H.A., Zulqarnain M., Ammar Y.A., Tan E.C., Rahman N.A. and Yusof R., 2014, Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2BNS3 protease assay, Tropical Biomedicine, 31 (2), 286–296. Safitri I., Yulis I.M. and Dasni H., 2009, Isolasi dan Uji Aktifitas Antimikroba Ekstrak Metanol Bunga, Batang dan Daun Sapu Jagad (Isotoma Longiflora (L) Presl.) terhadap Staphylococcus Aureus, Jik, 3 (1), 20–23. Saifudin A., 2014, Senyawa Alam Metabolit Sekunder, Deepublish, Sleman. Setyowati W.A.E., Ariani S.R.D., Ashadi, Mulyani B. and Rahmawati C.P., 2014, Skrining Fitokimia Dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk, Seminar Nasional Kimia dan Pendidikan Kimia VI, 271–280. Siregar R.M., 2015, Antibacterial Activity of Chitolod (Laurentia longiflora (L). Peterm) Leaf and Flower Extact Against Several Conjunctivity Causing Bacteria, Bogor Agricultural University, 1 (L), 8. Suhendi A., Muhtadi, Leonita Adhiyati H, Sudjono T.A. and Haryoto, 2014, Aktivitas Sitotoksik dari Ekstrak Kulit Buah Durian (Durio zibethinus Murr.), dan Kelengkeng (Dimocarpus longan Mark.) terhadap Sel Vero dan HeLa, 59–63. Wardani T. and Siska H., 2010, Uji Efek Antiglaukoma Infus Daun Chitolod (Isotoma longiflora (L) C. Presl) Terhadap Tikus Putih Jantan Berdasarkan Tekanan Bola Mata, Ejournal.Uhamka.Ac.Id, (L), 5. | id_ID |
| dc.description.abstract | Breast cancer is a major cancer suffered and the most cause of death by women in the world. The
existing drugs of anticancer are not selective, disturbing the growth of normal cells. It encourages
researchers to explore a chemopreventive agent from the nature materials. This study was conducted
to determine the cytotoxic activity of ethanol extract, polar fractions, semipolar, and nonpolar herb
chitolod (Isotoma longiflora (L) Presl.) on MCF-7 cells. First, herb chitolod was dried, refined, and
sieved using mesh number 40 and then macerated using 96% ethanol for 72 hours. Phytochemical
screening was conducted with TLC using GF254 silica as stationary phase, n-hexane: ethyl acetate (7: 3)
as mobile phase, then the result was visualized on UV 254 nm, 366 nm, and visible light. Anisaldehid,
dragendroff, sitoborat, and FeCl3 reagents spray were used as visualizer of TLC spot results. Fractination
using KCV method, then cytotoxic test using MTT assay. A result obtained, the ethanol extract of
herb chitolod positively contains flavonoids, phenolics, alkaloids, terpenoids, saponins, steroids and
tannins. IC50 value of the ethanol extract, polar fractions, semipolar, and nonpolar are 521,05; 213,29;
499,94; and 239,43 μg/mL. This study showed that the ethanol extract, polar fraction, semipolar, and
nonpolar of herb chitolod had moderate cytotoxic activity on MCF-7 cells. | id_ID |
