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Anticancer Activity of Methanol and Hexane Extract of Pereskia grandifolia Haw Leaves Against Human Cervical (HeLa) Cells Line

dc.contributor.authorKarim, Aditya Krishar
dc.contributor.authorSismindari
dc.date.accessioned2012-12-12T05:01:43Z
dc.date.available2012-12-12T05:01:43Z
dc.date.issued2012-06
dc.identifier.citationAziz, M.F., Andrijono, dan Saifuddin, A.B., 2006, Onkologi dan Ginekologi Buku Acuan Nasional. Yayasan Bina Pustaka Sarwono Prawirohardjo. Jakarta. Butterworth, C and Edwards, E.J., 2008, Investigating Pereskia and The Earliest Divergences in Cactaceae, Haseltonia., 14, 46-53. Edwards, E.J., Nyffeler, R., and Donoghue, M.J., 2005, Basal Cactus Phylogeny: Implications of Pereskia (Cactaceae) Paraphyly for The Transition to The Cactus Life Form, American J. Bot., 92, 1177-1188. Er, H.M., Cheng, En-Hsiang., and Radhakrishnan, A.K., 2007, Anti- Proliferative and Mutagenic Activies of Aqueous and Methanol Extract of Leaves from Pereskia bleo (Kunth) D.C (Cactaceae)., J.Etnopharm., 113, 448-456. Goh, K.L., 2000, Malaysian Herbacous Plants. Milllennium edn. Advanco Press. Malaysia. Newman, D.J., and Gragg, G.M. 2007. Natural product as Sources of New Drugs Over the Last 25 Years, J.Nat.Prod., 70, 461-477. Sri Nurestri, A.M., Wahab, N.A., Yacob, H., Shin, S.K., Lai, H.S., Serm, L.G., and Rahman, N.S.A., 2008, Cytotoxic Activity of Pereskia bleo (Cactaceae) Againts Human Cell Lines. Inter.J.Cancer.Res., 4(1), 20-27. Sri Nurestri, A.M., Sim, K.S., and Norhanom, A.W., 2009, Phytochemical and Cytotoxic Investigations of Pereskia grandifolia Haw. (Cactaceae) Leaves, J.Bio. Scie., 9(5), 488-493. Sim, K.S., Sri Nurestri, A.M., and Norhanom, A.W., 2010, Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts, Pharmacogn.Mag., 6(23), 248-254. Puspitasari, H.P., Sukardiman, and Aty, W., 2003, Uji Aktivitas Sitotoksik Ekstrak Metanol Herba Ageratum conyzoides L Pada Kultur Sel Mieloma Mencit, Majalah Farmasi Airlangga., 3(3), 93-96. Ramli, M., Rainy, U., and Sonar, S.P., 2005, Deteksi Dini Kanker. Eds. Balai Penerbit FKUI. Jakarta. Rasjidi, I., 2007, Panduan Penatalaksanaan Kanker Ginekologi Berdasarkan Evidance Base. Penerbit Buku Kedokteran EGC. Jakarta. Tan, M.L., Sulaiman, S.F., Najimuddin, N., Samian, R.M., and Muhammad, T.S., 2005, Methanolic Extract of Pereskia bleo (Kunth) D.C. (Cactaceae) nuces Apoptosis in Breast Carcinoma, T47D Cell Line, J.Ethno.pharm., 96, 287-294. Takeiti, C.Y., Graziella, C., Antonio, G.C., Motta, E.M.P., Fernanda, P.C.Q., and Park, K.J., 2009, Nutritive Evaluation of a Non-Conventional Leafy Vegetable (Pereskia aculeata Miller), Inter.J.Food.Scie.Nutr., 60(1), 148- 160. WHO, 2002. Cervical Cancer Screening in Developing Countries : Report of a WHO Consultation. WHO Press, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland. 75p. Wahab, S.I.A., Abdul, A.B., Mohan, S.M., Al-Zubairi, A.S., Elhassan, M.M., and Ibrahim, M.Y., 2009, Research Article Biological Activities of Pereskia bleo Extracts, Inter.J.Pharm., 5(1), 71-75.en_US
dc.identifier.isbn978-602-18711-0-2
dc.identifier.urihttp://hdl.handle.net/11617/2297
dc.description.abstractPereskia grandifolia Haw belongs to the Cactaceae family. This plant is claimed by local community in Malay to have medicinal properties to treat a variety of illnesses including diabetes, gastric pain, cancer, anti-inflammation, ulcer and hypertension. The objective of this research is to examine the anticancer activity of P. grandifolia leaves methanol and hexane extract against cervical cancer cell line (HeLa) culture. Leaves powder were extracted using methanol and hexane. Anticancer activity was measured by MTT [3- (4,5-dimetilthiazol-2-il)-2,5-difeniltetrazolium bromida) assay method. The result indicated that P. grandifolia leaves methanol extract possessed low anticancer activity against HeLa cell line with the IC50 value of 826.13 µg/ml, 928.42 µg/ml 899.90 µg/ml respectively and leaves hexane extract with the IC50 values of 816.85 µg/ml, 1,416 µg/ml, and 749.70 µg/ml respectively after incubated 24, 48 and 72 hours. The result indicated the possible use of medical plant, P. grandifolia as a source of chemopreventive agents.en_US
dc.language.isoenen_US
dc.publisherMuhammadiyah University Pressen_US
dc.subjectPereskia grandifolia Hawen_US
dc.subjectcytotoxicen_US
dc.subjectHeLa cellen_US
dc.subjectMTT assayen_US
dc.titleAnticancer Activity of Methanol and Hexane Extract of Pereskia grandifolia Haw Leaves Against Human Cervical (HeLa) Cells Lineen_US
dc.typeArticleen_US


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