Antiproliferation Activity of Nigella Sativa on 7,12dimethylbenz[A] Antracene Induced Carcinogenesis in Female Mice Breast

Abstract

Breast cancer is the leading cause of death in the world. Nigella sativa is one of the plants that has anticancer activity. Previous study reported Nigella sativa has cytotoxic activity in T47D cell. This study aimed at observing the effect of cloroform extract of Nigella sativa seed (NSS) on mice breast cell after initiation of 7,12-dimethylbenz [a] anthracene. Sprague Dawley strain female rats were divided into five groups. Each group consisted of 12 mice. The experiment consisted of five mice groups, corn oil solvent control group, the DMBA dose 20 mg/kgBW p.o. twice a week during five weeks, DMBA+NSS dose 250 mg/kgBW, DMBA+NSS dose 500 mg/kgBW and DMBA+NSS dose 750 mg/kgBW. Extract dissolved in corn oil was administered daily by the oral route 1 week before and during the DMBA induction. At the end of the study, mice was sacrified and breast organs were collected and stained with Haematoxylin and Eosin (H&E) and AgNOR methods. H&E staining showed that there were no histopatology changes in treated group compared with DMBA group. NSS was able to supress breast cancer cell proliferation activity. mAgNOR value on DMBA control group, dose 250, 500, 750 mg/Kg BW NSS+DMBA and corn oil were 2.14 ± 0.33; 1.47 ± 0.55; 0.83±0.24; 0.33 ± 0.23; 0.22±0.19, respectively. The results suggested that N.sativa can be develop into chemopreventive agent for breast cancer.