PENGARUH LAMA PRAPERLAKUAN FLAVONOID RUTIN TERHADAP EFEK HIPOGLIKEMIK TOLBUTAMID PADA TIKUS JANTAN YANG DIINDUKSI ALOKSAN

Abstract

Rutin, a flavonoid is known as cytochrome P450 inhibitor that play role in tolbutamid metabolism. In diabetic patients, oxidative stress is increased, so they need antioxidant from outside of the body. Rutin use as dietary supplement which has activities as antioxidant. The aim of this study is to know the influence of Rutin againts hipoglycaemic effect of tolbutamid in alloxan-induced diabetic rats. Fifteen male Sprague Dawley rats, aged about 2-3 months and weight 150-250 g which were induced by alloxan 150 mg/kgBW intraperitoneal (diabetic rats), divided into five groups, each group contains 3 rats. Group I (control diabetics) without treatment, group II was given CMC Na 1%, group III (positive control) was given suspension of tolbutamid 360 mg/kgBW, groups IV and V were given pretreatment of Rutin 45 and 180 mg/kgBW orally respectively an hour before treatment of tolbutamid 360 mg/kgBW. Blood is taken from tail lateralis vein at 60 minute before treatment, 30; 60; 120 and 180 minutes after treatment of tolbutamid. The increased of data AUC30-180 from percentage blood glucosa level was analysed by one way Anava and continued by LSD test with level of confident 95%. The results showed that pretreatment of Rutin dose 45 and 180 mg/kgBW were not influenced the hypoglycaemic effect of tolbutamid 360 mg/kgBW (p>0,05)