Antidiabetic Drug Ethyl Acetate Fraction of Leucaena leucocephala Seed Extract in Wistar Aloxan Induced
Chahyono, Tri Bowo
Syarifah, Andiri Niza
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Diabetes mellitus (DM) is a disease with high prevalence in Indonesia. According to data of Republic Indonesia Healthy Ministry Department in 2008, the prevalence of DM in Indonesia is 5.7 % and WHO also predicts in 2025 Indonesia will be the fifth biggest country for diabetics in the world. Leucaena leucocephala is used empirically as antidiabetes, anticholesterol and intestinal worms. Flavonoid compounds in the seed were thought to reduce insulin production. The aim of this research was to determine the effect of ethyl acetate fraction from Leucaena leucocephala extract to reduce blood sugar levels in alloxanmonohydrate-induced hyperglycaemic wistar rats. The extract was prepared by maceration of dried and powdered seeds of L. leucocephala using ethanol 70 % and it was then fractionated with ethyl acetate. The experiment was carried out by controlled group post-test method using 24 male wistar rats, divided into 6 groups, i.e. A = normal control, B = negative control (alloxan), C = positive control (alloxan+glibenclamide), D = (alloxan+ethyl acetate fraction of L. leucocephala extract 100mg/kgBW), E = (alloxan+ethyl acetate fraction of L. leucocephala extract 250mg/kgBW) and F = (alloxan+ethyl acetate fraction of L. leucocephala extract 500mg/kgBW). The results were analyzed with ANOVA and post hoc test statistical assay. The results of the research showed that the ethyl acetate fraction of Leucaena leucocephala seed extract had an effect to decrease the rats glucose blood level in the 100 mg/kg BW dosage and 500 mg/kg BW can decrease the glucose blood level (P<0,05) more than the negative control is (235.00 ± 54.903) and (240.28 ± 34.794) mg/dL.