Efek Antioksidan Ekstrak Etanol 70% Biji Alpukat (Persea Americana Mill) dengan Metode DPPH

Abstract

Avocado is traditionally used to treat various diseases among others, menorrhagia, diabetes, diarrhea, hypertension and others. In everyday use avocado seed is often not utilized. This study aims to know the effects of antioxidant of 70% ethanolic extract of the avocado seed (Persea Americana Mill). The method used is the in vitro antioxidant with DPPH. The extract was dissolved in methanol and made in a variety of concentrations, namely 10, 30, 50 and 70 ppm respectively as much as 10 ml. Into each solution was added to 1 mL of 1 mM DPPH solution and incubated at 37 ° C for 30 minutes, then measured at a wavelength of 515 nm. As the blank is used methanol and DPPH 1mM. BHT (butylated hydroxytoluene/BHT with concentration of 2 , 4 , 6 , 8 ug / mL were used as a comparator The results show the 70% ethanolic extract of avocado seed can inhibit free radicals with IC 41,5ppm.

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