Antibacterial Activity of 3,6-Bis [(4-Hydroxy-3,5-Dimethylphenyl)Methylidene] Piperazine-2,5-Dione against Staphylococcus aureus, Escherica coli and Bacillus subtilis

Abstract

Curcumin has antibacterial activity. Modification of curcumin to obtain a more potent compound, stable, safe, effective, and has a better activity has been widely applied. Some of them are derived compounds of pentagamavunon (PGV) and derivatives of piperazine-2,5-dione. Molecule of 3,6-bis[(4-hydroxy-3-methoxy)metiliden]piperazine-2,5-dione (DKP1) and 3,6-bis[(4-hydroxy-3,5-dimetilphenyl)metiliden]piperazine-2,5- dione (DKP2) are derivates of piperazine-2,5-dione. This study aims to determine the antibacterial activity of DKP1 and DKP2 against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The antibacterial assay was done using Kirby Bauer diffusion method. Dics were impregnated with curcumin, PGV-0, PGV-1, DKP1 and DKP2. The discs were place on Mueller Hinton media that has been inoculated with the test bacteria. Antibacterial activity was determined by measuring inhibition zone around the discs. The results showed that DKP2 has weak antibacterial activity against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. Pentagamavunon-0 (PGV-0) has activity against E. coli and along with PGV-1 against Staphylococcus aureus. Curcumin and DKP1 have no antibacterial activity against Staphylococcus aureus, and along with PGV-0 and PGV-1 against Bacillus subtilis.