Effectiveness Of Ethanolic Extract Of Sarang Semut (Myrmecodia tuberosa (non Jack) BI.) to Decrease Blood Glucose Levels In Diabetic Rats Induced by Alloxan

Abstract

Sarang semut (Myrmecodia tuberosa (non Jack) BI.) contain active compounds such as flavonoids, tannins, polyphenols, tocopherols, and minerals Flavonoid in Sarang semut suspected can lower blood glucose levels by mechanism of stimulating insulin-secreting pancreatic β cells. The purpose of this study was to determine the effect of ethanolic extract of sarang semut to decrease blood glucose levels in rats induced by alloxan. This research used pretest and posttest with control design method. Before treatment, blood was taken from tail vein lateralis rats to measurement of glucose levels (baseline). Later, rats were induced by alloxan 160 mg/kgBW (intraperitoneal) to make diabetic in rats. Three days later after induced by alloxan, blood glucosa was measured. Twenty male of rats with glucose levels ≥ 200 mg/dL were used to research (as pretest) and divided into 5 groups. The first group (negative control) was treated with CMC Na 0,5%. Second group was treated with glibenclamide 0,5 mg/kgBW (positive control). The third to fifth groups were treated with ethanolic extract of sarang semut with dose 400 mg/kgBW, 800 mg/kgBW, and 1600 mg/kgBW. The blood were taken on days 0, 3, 5,10,14 and 21(since pretest). The serum added to reagen kit glucose GOD FS from Diasys and readable the glucose levels used a spectrophotometer visible (λ 500 nm). Blood glucose levels were tested by ANOVA followed by LSD Post Hoc test. The result showed that ethanolic extract of Sarang semut with dose 400; 800 and 1600 mg/kgBW respectively could reduce significant (p<0,05) the blood glucose level and they had similar effect compared with positive control.