Sub-Cloning of ads Gene Into pETDUET1_cyp For Co-Expression in Escherichia coli
Wicaksono, Imam Adi
Ulfa, Evi Umayah
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CYP71AV1 and ADS are two enzymes involved in artemisinin biosynthesis. In this research, sub-cloning of cyp71av1 and ads in pETDUET1 (pETDUET1_cyp/ads) has been done. The result of transformation has been confirmed by migration, restriction and sequencing analysis. Overproduction of CYP71AV1 and ADS was done at temperature 37 °C using 0,5 mM IPTG induction. The protein produced mostly formed as inclusion bodies, therefore the optimization of overproduction condition is still needed.