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dc.contributor.authorIrjayanti, Anis Novida
dc.contributor.authorSuhendi, Andi
dc.contributor.authorSujono, Tanti Azizah
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dc.description.abstractEthanol extract of the leaves salaplant (CynometraramifloraLinn.) have cytotoxic activity against WiDr cells with IC50 of 6.37 μg/mL. This study aims to determine the cytotoxic activity of polar, semipolar, and nonpolar fractions of ethanol extract of leaves sala plant (CynometraramifloraLinn.) against WiDr cells. Fractionation was performed on Vacuum Liquid Chromatography method with silica G 60 as stationary phase and n-hexane: ethylacetate(8:2; 7.5:2.5; 7:3,6:4 and 3:7) and ethanol as mobile phase. Qualitative screening of major compounds used TLC method with spray reagent. Cytotoxicity determination performedin vitro model cell lines with MTTreagent.The result showed that IC50 of polar, semi polar and non polar fractions were 231.95 μg/mL and cannot determined. Doxorubicin was used as positive control and obtained IC50 of 1.721 μg/mL.in_ID
dc.publisherUniversitas Muhammadiyah Surakartain_ID
dc.subjectMTT assayin_ID
dc.subjectCynometra ramiflorain_ID
dc.subjectWiDr cellin_ID

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