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dc.contributor.authorchakim, Irfanul
dc.date.accessioned2016-02-27T13:38:55Z
dc.date.available2016-02-27T13:38:55Z
dc.date.issued2015-08
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dc.identifier.issn2407-9189
dc.identifier.urihttp://hdl.handle.net/11617/6844
dc.description.abstractEntomological study with laboratory analysis done on Anopheles sundaicus mosquitoes as a malaria vector. The aim is to determine the existence of genetic variation in the Anopheles sundaicus species. PCR was used for the molecular identification of An. Sundaicus using COI and ITS2 genes. COI and ITS2 sequences in the study group compared with An. Epiroticus from GenBank. The comparation were done using a multiple sequence alignment program of Bioedit 7.1.9. The phylogenetic tree were constructed using neighbor-joining and bootstrap- tested by MEGA6. ITS2 sequence alignment results showed a single nucleotide substitution that can be used as markers for species determination. Phylogenetic tree analysis based on COI gene showed that An. Sundaicus in this study with isolates from GenBank, separate into two different groups or are polyphyletic. This study proved that An. Sundaicus from field collection was An. Epiroticus species by molecular identification. This study can be used to help public health policy related to vector control.in_ID
dc.language.isoidin_ID
dc.publisherLPPM Universitas Muhammadiyah Semarangin_ID
dc.subjectPCRin_ID
dc.subjectCOIin_ID
dc.subjectITS2in_ID
dc.subjectAn. Sundaicusin_ID
dc.subjectAn. Epiroticusin_ID
dc.titleVariasi Genetik Anopheles Sundaicus Berdasarkan Segmen ITS2 DNA Ribosom dan Gen Coi DNA Mitokondriain_ID
dc.title.alternative(Genetic Diversity In Anopheles Sundaicus Using ITS2 Ribosomal DNA Region and Coi Mitochondrial DNA Region)in_ID
dc.typeArticlein_ID


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