Pengaruh Konsentrasi Ekstrak Etanol Buah Delima (Punica granatum Linn.) terhadap Peningkatan Apoptosis Sel Kanker Lidah Manusia Sp-C1 In Vitro

Abstract

Squamous cell carcinoma is one of the most common malignancy in the oral cavity. Currently, cancer treatment using surgery, radiotherapy, chemotherapy and combination. New strategy for cancer therapy is by inducing apoptosis using herbal medicine. Pholyphenol is one of a potential anticancer agent that can induce apoptosis. Pomegranate is one of a potential herbal medicine because of its pholyphenol enzyme content. The aim of study was to investigate to relation between pomegranate ethanol extract and apoptosis of oral tongue squamous cell carcinoma cell line (SP-C1). The true experimental method was conducted in the present study. Oral tongue squamous cell carcinoma cell line (SP-C1)was treated with three concentration under and upper IC50 and upper of pomegranate ethanol extract, including 25, 50, 75, 100, 150, 200, 250µg/ml. In apoptosis test, cell stained with fluorochrome ethidiun bromide and acridine orange after 24 hours incubation. Fluorescence microscope was use for counting the cell. Viable cell would be stained green and apoptotic cell would be stained yellow. Data was analyzed by Saphiro-Wilk test and Levene test to determine normality and homogeneity of data. If data distribution was normal and homogen, analyze were continue by Parametric test: Pearson correlation, linier regression and One Way ANOVA.The result showed that there was a strong positive correlation between concentration of pomegranate ethanol extract and apoptotic cell (r = 0,984, p< 0,05). Pomegranate concentration was a predictor of apoptosis induction 98,6 %. One Way ANOVA test revealed there were a statistically significant difference between each group treated with different concentration (p<0,05). In conclusion, pomegranate ethanol extract treatment induced apoptosis of oral tongue squamous cell carcinoma and the increases of pomegranate ethanol extract concentration followed by the increase of apoptosis induction.