Influence Dried Flower of Hibiscus sabdariffa Linn Infusion on Serum Glutamate Pyruvate Transaminase (SGPT) Level against Paracetamol Induced Liver Injury in Rats

Abstract

The level of Serum Glutamate Pyruvate Transaminase (SGPT) could be elevated higher than normal due to hepatocelluler necrosis caused by virus or hepatotoxin such as paracetamol given in toxic dose. The aim of this research was to determine the ability of dried flower of Hibiscus sabdariffa Linn infusion to decrease the level of SGPT in male rats which were induced by toxic dose of paracetamol. This study used 25 Wistar male rats of 3-4 month old, which were randomly divided into 5 groups. Group I (normal control) and Group II (negative control) were given aquadest for 7 consecutive days, groups III until V were given the infusion of Hibiscus sabdariffa Linn with concentration of 10%, 20%, and 40% b/v; 2.5 ml/kg BW respectively for 7 consecutive days. Twenty four hours after the last treatment, group II until V were induced liver injury by toxic dose of paracetamol 2.5 g/kg BW and after 36 hours, the blood was taken from the tail vein lateralis and the SGPT activity were measured by GPT ALAT method. The result showed that the administered infusion of Hibiscus sabdariffa Linn 20 and 40 % could decrease SGPT significantly to become 388.8 ± 18.79 (67.74%) and 172.2 ± 87.48 U/L (85.55%), whereas the negative control was 1190 ± 443 U/L. Infusion of Hibiscus sabdariffa Linn concentration 20 and 40% have hepatoprotective effect against toxicity of paracetamol.