Antidiabetic Drug Ethyl Acetate Fraction of Leucaena leucocephala Seed Extract in Wistar Aloxan Induced
Date
2012-06Metadata
Show full item recordAbstract
Diabetes mellitus (DM) is a
disease with high prevalence in Indonesia. According to
data of Republic Indonesia Healthy Ministry Department in 2008, the prevalence of DM in
Indonesia is 5.7 % and WHO also predicts in 2025 Indonesia will be the fifth biggest country
for diabetics in the world. Leucaena leucocephala is used empirically as antidiabetes,
anticholesterol and intestinal worms. Flavonoid compounds in the seed were thought to
reduce insulin production. The aim of this research was to determine the effect of ethyl
acetate fraction from Leucaena leucocephala extract to reduce blood sugar levels in alloxanmonohydrate-induced
hyperglycaemic
wistar
rats.
The
extract
was
prepared
by
maceration
of
dried
and
powdered
seeds
of
L.
leucocephala
using
ethanol
70
%
and
it
was
then
fractionated
with
ethyl
acetate.
The
experiment
was
carried
out
by
controlled
group
post-test
method
using
24
male wistar rats, divided into 6 groups, i.e. A = normal control, B = negative control
(alloxan), C = positive control (alloxan+glibenclamide), D = (alloxan+ethyl acetate fraction
of L. leucocephala extract 100mg/kgBW), E = (alloxan+ethyl acetate fraction of L.
leucocephala extract 250mg/kgBW) and F = (alloxan+ethyl acetate fraction of L.
leucocephala extract 500mg/kgBW). The results were analyzed with ANOVA and post hoc test
statistical assay. The results of the research showed that the ethyl acetate fraction of
Leucaena leucocephala seed extract had an effect to decrease the rats glucose blood level in
the 100 mg/kg BW dosage and 500 mg/kg BW can decrease the glucose blood level (P<0,05)
more than the negative control is (235.00 ± 54.903) and (240.28 ± 34.794) mg/dL.