Perbandingan Efek Ekstrak Eter dengan Ekstrak Etanol Biji Tua Pisang Kluthuk (Musa balbisiana Colla) pada Sekresi Asam Lambung Tikus Putih in vitro

Abstract

Kluthuk banana (musa balbisiana colla), a seeded banana, has been studied for its ability to reduce gastric acid secretion. Is the active compound hydrophylic or hydrophobic has not been known yet.The aim of his study is to determine the effect of the seed of ripe kluthuk banana (musa balbisiana colla) etheric and ethanolic extract, and to compare the effect of both extract on rat gastric acid secretion in vitro. This study was conducted using isolated Wistar rat stomach, according to the methods modified from Barocelli. Fifty four rats (3-4 month) of 150-250 g were used in this study. They were divided into 9 groups (6 rats each). The rats were fasted and drinking water was given ad libitum for 24 hours before testing. The rats were weighted, anesthetized with ether inhalation, and then sacrified. The stomach was taken and suspended in an organ bath containing 37°C buffered serosal solution and bubbled with carbogen. The gastric lumen was perfused continuously with unbuffered mucosal solution 1 ml min-1 and bubbled with 100% O2. The isolated preparation was stabilized for 1 hour and perfusate spilled out. The perfusate + was allowed to flow continuously, and collected for 10 minutes duration. The H consentration was measured by + titration with NaOH 0,002 N using phenolftalein as indicator, known as basal H consentration . Pretreatment was added to the unbuffered mucosal solution for 30 minutes, after which the treatment (histamine 10-6 M) was added to the unbuffered solution in each group. Perfusate from gastric lumen were collected every 10 minutes untill 80 + + minutes and H consentration were measured by mean of titration. The H consentration elevation was expressed as mean ± SEM, the AUC0-80 was calculated and analyzed by ANOVA.The result showed that the total area under + curve (AUC0-80) of H consentration was 14550 ± 692,70 in control group (saline), 141,67 ± 2838,62 in etheric extract of the seed of ripe kluthuk banana doses-1 group (EESRKB I group) equivalent to 1,92 mg/kgBW, 8516,67 ± 3659,64 in EESRKB II group (equivalent to 3,84 mg/kgBW), 5650 ± 3191,94 in EESRKB III group (equivalent to 7,69 mg/kgBW), 4500 ± 2819,22 in ethanolic extract of the seed of ripe kluthuk banana doses-1 group (AESRKB I group) equivalent to 8,7 mg/kgBW, -5883,33 ± 760,45 in AESRKB II group (equivalent to 17,4 mg/kgBW), -1116,67 ± 3444,36 in AESRKB III group (equivalent to 34,8 mg/kgBW), -1333,33 ± 80,28 in cimetidin group, and 12683,33 ± 968,65 in DMSO group. The statistical analysis showed that the AUC0-80 of EESRKB and AESRKB were significantly (p<0,05) lower than those of control solution (except EESRKB II), the AUC0-80 of EESRKB I were not significantly different (p>0,05) than those of AESRKB I, the AUC0-80 of EESRKB II were significantly higher (p<0,05) than those of AESRKB II, and the AUC0-80 of EESRKB III were not significantly different (p>0,05) than those of AESRKB III. It was concluded that the etheric and ethanolic extract of the seed of ripe kluthuk banana (Musa balbisiana Colla) showed an inhibitory effect on rat gastric acid secretion induced by histamine, and the ethanolic extract showed an inhibitory effect more than those of etheric extract.