Comparison of Antibacterial Activity of Ethanolic Extracts of Seed and Stem Papaya (Carica Papaya l.) Against Shigella Dysenteriae and Streptococcus Pyogenes

Abstract

Generally, seed and stem of papaya (Carica papaya L.) are not used. In a previous study, seed and stem of papaya inhibit growth of Escherichia coli and Staphylococcus aureus. The aim of this study was to compare antibacterial activity of ethanolic extract of papaya seed to stem extract against Shigella dysenteriae and Streptococcus pyogenes. Papaya seed and stem were extracted using 70% ethanol by maceration method. The antibacterial activity tests were carried out using Kirby-Bauer disk diffusion method. Chemical compounds in the extracts were identified using thin layer chromatography (TLC) with silica GF254 as stationary phase and a mobile phase of ethyl acetate : methanol : water (100 : 13 : 17)v/v for papaya seed extract and methanol:chloroform (8:2)v/v for papaya stem extract. The results showed that ethanolic extract of papaya seed and stem have same antibacterial activity against Shigella dysenteriae and Streptococcus pyogenes. Based on TLC analysis, papaya seed contain alkaloid, steroid, and tannin, while papaya stem contain tannin.