Uji Aktivitas Antimikroba Ekstrak Etanol Daun Cocor Bebek (Kalanchoe pinnata) terhadap Bakteri Staphylococcus aureus Atcc 6538 dan Escherichia coli Atcc 11229 Secara Invitro

Abstract

Cocor Bebek leaves (Kalanchoe pinnata) contains cinamic acid, flavonoid, alphatocopherol dan bufadienolide acid which are presumably able to impede a bacterial growth so that the ethanol extract of cocor bebek leaves are indicated having an antimicrobe effect. This research purposes to find out the existence and nonexistence of the impeding power of the ethanol extract of cocor bebek leaves (Kalanchoe pinnata) on the Staphylococcus aureus and Escherichia coli bacterias growth. The research is laboratory experimental with the ethanol extract of cocor bebek leaves (Kalanchoe pinnata) as the research subject. Bacterias which are used are Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 11229. The research method is Kirby Bauer by using an oxoid disk. The first step is standardizing each of 24 hours-aged Staphylococcus aureus dan Escherichia coli on BAP and Mc. Conkey medias in the standard of 0.5 Mc.Farland, then smearing using a sterile cotton-rid on Muller Hinton media. The researcher uses an empty oxoid disk as a negative control, an amoxicillin antibiotic disk on Staphylococcus aureus and a chloramphenicol on Escherichia coli as a positive control, while the researcher also places the oxoid disk containing the ethanol extract of cocor bebek leaves (Kalanchoe pinnata) with 20%, 40%, 60%, 80% and 100% concentrations on the top of the plates. Then the researcher measures the impeding zone which is formed after the incubation on 370°C for 1x24 hours. After that, the researcher analyzes the data using Mann-Whitney Non Parametry Test. The result is that on the degrees of 80% and 100%, Staphylococcus aureus bacteria has a significant difference (p<0,05) from the positive and negative controls. In conclusion, this research proves the existence of the antibacteria effect of the ethanol extract of cocor bebek leaves (Kalanchoe pinnata) on the Staphylococcus aureus growth in the concentrations of 80% and 100% and the nonexistence of the antibacteria effect on the Escherichia coli growth.