Potential of Salam Leaves (Syzigium polyanthum Walp.) and Habatus Sauda Seeds (Nigella saliva Linn.) as Anti-uric Acid Herbal Medicine Materials; Study of Preclinical Testing and Extract Standardization

Abstract

Antihiyperuricemia activity testing has been carried out in vivo from a single and combination of extracts from salam leaves tSyzigium polynthum Walp) and habatussauda seeds (Ambonicus Coleus Lour.) Extraction of salam leaves and habatussauda seeds had been done by boiling its with water until the volume becomes a half, then filtered and pressed. The filtrate was dried by evaporation with the helping of waterbath and vacuum oven. Dried extracts of each ingredient, then proceed antihiyperuricemia preclinical testing in vivo on male white mice Balb-C strain that has been induced by potassium oxonic dose of 250 mg/kg. The results of antihyperuricemia preclinical testing showed that extracts of salam leaves and habatussauda seeds single dose of 200 mg/kg had lower activity levels of uric acid in blood serum of male white mice, respectively, 0.520 and 1.180 mg/dL. While antihyperuricemia activity of a combination of salarn-habatussauda extracts is 0.900. Each of the extracts had been analyzed for standardization extract with the test procedure based on the parameters of Materia Medica Indonesia and the general standardize of medicinal plant extracts suggested by BPOM RI, which include non- specific and specific parameters. Overall, based on the results of standard tests common medicinal plant extracts, the two materials under study had met the recommended requirements.