Antioxidant, Cytotoxic, Antihyperuricemic Activities and Chemical Constituents of Some Indonesian Fruit Peels

Abstract

This study was designed to investigate the antioxidant, cytotoxic, antihyperuricemic activities and chemical constituent of the ethanolic extracts of some Indonesian fruit peels, namely Durian, Rambutan, Jeruk Manis and Kelengkeng. The peels of some Indonesian fruits had been extracted with the ethanol solvent and fractionated with the partition method, started n-hexane, chloroform, ethyl acetate and methanol-water solution. The antioxidant activities were evaluated by using DPPH radical scavenging assay. The strongest antioxidant activity was shown by the Rambutan peel extract, with IC50 values of 7.74 µg/mL, more active than vitamin E (IC50 = 8.48 ± 0.1 µg/mL). The cytotoxic activities were evaluated using MTT assay against HeLa, T47D and WiDR cell lines. The result of cytotoxic activities testing showed very weak against all cell lines tested. The antihyperuricemic activities were evaluated in mice Balb-C strain induced by potassium oxonate dose of 250 mg/kg body weight. The result of antihyperuricemic activities testing showed that the extracts of rambutan peel dose of 500 mg / kg bw had reduced strongest of uric levels acid in the blood serum of mice, with the value 91.81%, exceeds the activity shown by allopurinol 66.67%. The chemical constituents of Rambutan and Durian were successfully isolated using vacuum liquid chromatography and radial chromatography technique, and these structures were characterizated based on the proton (1H) and carbon (13C) NMR spectra. The isolated compounds were ethyl gallate (1) from Rambutan (Nephelium lappaceum Linn.) rind, 4,4-dimethyl- poriferasta-18(19)-en-3-ol (2) and 3α-E-ferulyloxy-lup-20(29)-en-28-oic acid (3) from Durian (Durio zibethinus Murr.) peels.