The Ethanol Extract of Physalis angulata Linn Inhibits COX-2 Activity in MCF-7 Cell In Vitro

Abstract

Some studies showed that ceplukan (Physalis angulata Linn) have cytotoxic effects toward HeLa (cervical cancer), KB (Nasopharyngeal), Colo 205 (colon), Calu (Lung) and MCF-7 cells in vitro. This plant has cytotoxic effects toward rat P388 lymphocytic leukemia in vivo. One of the mechanisms of cytotoxicity is the inhibition of Cyclooxygenase-2 (COX-2) pathway. The purpose of this study was to determine if 70% ethanol extract of Physalis angulata Linn inhibited COX-2 activity in MCF-7 cell. The cytotoxic effect of ethanol extract of Physalis angulata Linn toward MCF-7 cell was examined at concentration 20, 40, 80 and 160 µg/mL. Its estimated IC50 was used to test inhibition of COX-2 activity. After 24 hours of incubation, the inhibition of COX-2 activity was assayed by imunohistochemical staining with a monoclonal anti COX-2 antibody. COX-2 positive cells were counted using binocular microscope and IC50 for inhibition of COX-2 activity was calculated. The inhibition of COX-2 activity at 10, 20 and 40 µg/mL of the extract was 39.3%, 42.06% and 51.73%, respectively. The IC50 value of the ethanol extract of P. angulata Linn. for the inhibition of COX-2 activity was 37.57 ± 3.11 µg/mL, while the IC50 of celecoxib was 5.31 ± 0.27 µg/mL. The ethanol extract of Physalis angulata Linn. have an inhibitory effect on COX-2 activity in MCF-7 cell with IC50 37.57 ± 3.11 µg/mL