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dc.contributor.authorSutrisna, EM
dc.contributor.authorIndwianiastuti
dc.date.accessioned2012-12-12T04:09:37Z
dc.date.available2012-12-12T04:09:37Z
dc.date.issued2012-06
dc.identifier.citationAnonim1, 2005. Disease Specific NCD Morbidity and Mortality Profile, Http://w3.whosea.org/linkfiles/NCD_i nforbase.disease-spesific.pdf, diakses, April 2005 Anonim2,1998, Feasibility Demonstration Project for HTPS, Http://www. bdbiosciences.com/ clontech, diakses Juli 2005 Bakhriansyah, M., 2004. Pengaruh Ekstrak Etanol Biji Mahkota Dewa pada Sel Kanker Payudara T47D: Kajian Aktivitas Sitotoksik, Antiproliferatif dan Penghambatan Ekspresi Siklooksigenase- 2, Thesis, Fakultas Pascasarjana UGM Jogjakarta. Chiang¹,H.C., Jaw SM, Chen CF, Kan WS, 1992. Antitumor Agent, Physalin F from Physalisangulata L., Anticancer Res., 12(3): 837-43. Chiang²,H.C., Jaw SM, Chen PM., 1992. Inhibitory Effects of Physalin B and Physalin F on Various Human Leukemia Cells in vitro, Anticancer Res., 12(4): 1155-62. Choi, EM, Hwang JK, 2003. Investigations of Anti Inflammatory and Antinociceptive Activities of Piper Cubeba, Physalis angulata and Rosa hibrida, J Ethnopharmacol, 89(1): 171-5. Costa, C., Soares, R., Reis-Filho, J.S., Amendoeira, I and Schmitt, FC., 2002. Cyclooxygenase 2 Expression is Associated with Angiogenesis and Lymphonode Metastasis in Human Breast Cancer, J. ClinPathol, 55: 429-34 Davies, G, Martin, L.A., Sacks, N., Dowsett, M., 2002. Cyclooxygenase-2 (COX-2), Aromatase and Breast cancer: A Possible Role in for COX-2 Inhibitors in Breast cancer Chemoprevention, Ann Oncol. 13: 669-78. Davies, G., Salter, J., Hills, M, Ann- Martin, L. Sacks, N., Dowsett, M., 2003. Correlation between Cyclooxygenase- 2 Expression and Angiogenesis in Human Breast cancer, Clin cancer Res. 9: 2651-2656 Grosch, S., Tegeder, I., Neiderberger, E., Brautigam, L., and Geislinger, G., 2001. COX-2 Independent Induction of Cycle Cells Arest and Apoptosis in Colon cancer Cells by The Selective COX-2 Inhibitor Celecoxib, FASEB, J., 10 (15): 2742-44. Haris, R.E., Namboodiri, K.K., Farrar, W.B., 1996. Nonsteroidal Anti-inflammatory Drugs and Breast cancer, Epidemiology, 7: 203-205. Hu, K., Yu, C., Mineyama, Y., McCracken, J.D., Hillebrand, D.J., and Hasan, M., 2003. Inhibited Proliferation of Cyclooxygenase-2 Expressing Human Hepatoma Cells by NS- 398, A Selective COX-2 Inhibitor. Int. J. Oncol. 22: 757-63. Kishi, K., Petersen, S., Petersen, C., Hunter, N., Mason, K., Masferrer, J.L., Tofilon, P.J. and Milas, L., 2000. Preferential Enhancement of Tumor Radioresponse by a Cyclooxygenase-2 Inhibitor, Cancer Res. 60: 1326-31. Langman, M.J.S., Cheng, K.K., Gilman, E.A. and Lankankershire, R.J., 2000. Effect of Antiinflamatory Drugs on Overall Risk of Common Cancer: Case Control Study in General Practice Research Database. Br Med J. 320: 1642- 46. Li, J., Wang, X., Chen, F., Yu., J., and Luo, H., 2003. Nimesulide Inhibits Proliferation via Induction of Apoptosis and Cell Cycle Arrest in Human Gastric Adenocarcinoma Cell Line. World J. Gastroenterol, 9(5): 915-20. Mangan, Y., 2003. Cara Bijak Menaklukkan Kanker, Cetakan I, AgromediaPustaka, Jakarta, 28-44 MOH, 1986, Sedia Galenik, Jakarta Sivula, A.,Talvensaarimatilla, A., Lundin, J., Joensuu, H., Haglund, C., Ristimaki, A., and Turpeenniemi-Hujanen, T., 2005. Association of Cyclooxygenase- 2 and Matrix Metal-loproteinase- 2 Expression in Human Breast Cancer, Breast Cancer Res. and Treat., 89: 215-220. Tjindarbumi, D and Mangunkusumo, R., 2002. Cancer in Indonesia, Present and Future. Jpn J Clin Oncol., 32 (1): S17-S21. Van de velde, C.J.H., Bosman, F.T., Wagener, D.J.Th., 1999. Onkologi, ed 5, Terjemahan Aryono, Gadjah Mada University Press, Jogjakarta Wu, S. J., Teik, L. Ng., Chen, C. H., Lin, D. L., Wang, S. S., and Lin, C. C., 2004. Antihepatoma Activity of Physalis angulata and P. peruviana Extracts and Their Effects on Apoptosis in Human Hep G2 Cells, Life sciences, 74: 2061-73.en_US
dc.identifier.issn978-602-18711-0-2
dc.identifier.urihttp://hdl.handle.net/11617/2293
dc.description.abstractSome studies showed that ceplukan (Physalis angulata Linn) have cytotoxic effects toward HeLa (cervical cancer), KB (Nasopharyngeal), Colo 205 (colon), Calu (Lung) and MCF-7 cells in vitro. This plant has cytotoxic effects toward rat P388 lymphocytic leukemia in vivo. One of the mechanisms of cytotoxicity is the inhibition of Cyclooxygenase-2 (COX-2) pathway. The purpose of this study was to determine if 70% ethanol extract of Physalis angulata Linn inhibited COX-2 activity in MCF-7 cell. The cytotoxic effect of ethanol extract of Physalis angulata Linn toward MCF-7 cell was examined at concentration 20, 40, 80 and 160 µg/mL. Its estimated IC50 was used to test inhibition of COX-2 activity. After 24 hours of incubation, the inhibition of COX-2 activity was assayed by imunohistochemical staining with a monoclonal anti COX-2 antibody. COX-2 positive cells were counted using binocular microscope and IC50 for inhibition of COX-2 activity was calculated. The inhibition of COX-2 activity at 10, 20 and 40 µg/mL of the extract was 39.3%, 42.06% and 51.73%, respectively. The IC50 value of the ethanol extract of P. angulata Linn. for the inhibition of COX-2 activity was 37.57 ± 3.11 µg/mL, while the IC50 of celecoxib was 5.31 ± 0.27 µg/mL. The ethanol extract of Physalis angulata Linn. have an inhibitory effect on COX-2 activity in MCF-7 cell with IC50 37.57 ± 3.11 µg/mLen_US
dc.language.isoenen_US
dc.publisherMuhammadiyah University Pressen_US
dc.subjectPhysalis angulata Linnen_US
dc.subjectcyclooxygenase-2 (COX-2)en_US
dc.subjectMCF-7 cell lineen_US
dc.titleThe Ethanol Extract of Physalis angulata Linn Inhibits COX-2 Activity in MCF-7 Cell In Vitroen_US
dc.typeArticleen_US


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