Oil Fraction from Kaempferia galanga Alcoholic Extract Increases Apoptosis Activity in Mice Colon Cancer
Santoso, Bilal Subchan A.
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Ethyl p-methoxycinnamate effectively inhibit the growth of cancer through a mechanism related to cell cycle regulation and apoptosis induction. We determined Kaempferia galanga that containing ethyl p-methoxycinnamate can increase the apoptosis activity in colon cancer cells. This study was designed to investigate effect of oil fraction from Kaempferia galanga alcoholic extract on apoptosis activity in mice colon cancer. Dimethylbenz(a)anthracene (DMBA)-induced colon cancer in mice is a useful model to investigate the changes of epithelial cells that occur during colon cancer progression. Colon cancer model was induced six times once a week by oral DMBA 50 mg/kg body weight. Administration of three different doses ethyl p-methoxycinnamate of oil fraction are 7.8 mg/kg, 23.4 mg/kg and 78 mg/kg which given to models during 30 days. Data analysis of immunohistochemical preparations was obtained and processed with one-way ANOVA statistical test, followed with post hoc test. Results showed that mice which given samples test increases apoptosis activity in colon cancer epithelial cells. The most effective dose of oil fraction increases apoptosis activity is containing ethyl p-methoxycinnamate 23.4 mg/kg body weight of mice.