PENETAPAN KADAR ASAM ASETILSALISILAT DALAM SEDIAAN OBAT MEMANFAATKAN SINAR REFLEKTAN TERUKUR DARI BERCAK YANG DIHASILKAN DENGAN TLC SCANNER

Abstract

Acetosal is consumed by people as analgesic and anti-pyretic so high quality of the product is required. Hydrolysis result of this compound is acetyl salicylic acid which is detected by forming complex of color compound with iron(III). As mention above, the needs of new determination method for the compound which is simple, valid and mobile is necessary to perform by detecting reflectance light of spot of salicylic-iron complex compound using TLC Scanner. Validation is needed to get the method valid and could use as determination procedure. The step of laboratory work are perform by making external standard curve, hydrolyzing the acetosal, adding the iron reagent, putting up the spot, measuring the reflectance light, performing validation parameter, calculating and analyzing the data. The results of recovery 80%; 100%; 120% (RSD), repeatability, linearity, intermediate precision and LOQ value are 119,53%; 118,86%; 119,76% (1,18%; 2,92%; 0,47%); 2,079%, r=0,938; 0,270%; dan 0,647% respectively. The conclusion is this method may use as alternative method for determining acetosal.