Antioxidant Activity, Total Phenolic and Flavonoid Content of Ethanolic Extracts of Local Longan (Euphoria longan Lour.) Seeds and Rinds
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The antioxidant, total phenolics and flavonoid content of local Longan (Euphoria longan Lour.) seeds and rinds were determined. The antioxidant activity test of ethanolic extract, hexane, ethyl acetate and methanol-water fractions of local longan seeds and rinds were evaluated by DPPH (2,2-diphenyl-1-picrilhydrazil) method. The total phenolic contents and total flavonoid contents were assessed spectrophotometrically. The results showed that the fraction of ethanol extract, ethyl acetate and methanol-water of longan seeds and rinds had high antioxidant activity whereas the hexane fraction had lowest activity. The highest activity was indicated of the ethyl acetate fraction longan rinds (IC50 = 9.23 µg/ml), ethyl acetate fraction longan seeds (IC50 = 9.50 µg/ml), and wasn’t significantly differ with vitamin E (IC = 8.88 µg/ml). The total phenolic content of the ethanol extract of longan rinds and seeds as well as fractions ranged from 8.72 ± 0,64 to 692.85 ± 41.11 mg/g sample. Whereas the total flavonoid levels in the samples ranged from 80.64 ± 6.98 to 680.15 ± 40.94 mg/g sample.