Antioxidant Activity, Total Phenolic and Flavonoid Content of Ethanolic Extracts of Local Longan (Euphoria longan Lour.) Seeds and Rinds

Abstract

The antioxidant, total phenolics and flavonoid content of local Longan (Euphoria longan Lour.) seeds and rinds were determined. The antioxidant activity test of ethanolic extract, hexane, ethyl acetate and methanol-water fractions of local longan seeds and rinds were evaluated by DPPH (2,2-diphenyl-1-picrilhydrazil) method. The total phenolic contents and total flavonoid contents were assessed spectrophotometrically. The results showed that the fraction of ethanol extract, ethyl acetate and methanol-water of longan seeds and rinds had high antioxidant activity whereas the hexane fraction had lowest activity. The highest activity was indicated of the ethyl acetate fraction longan rinds (IC50 = 9.23 µg/ml), ethyl acetate fraction longan seeds (IC50 = 9.50 µg/ml), and wasn’t significantly differ with vitamin E (IC = 8.88 µg/ml). The total phenolic content of the ethanol extract of longan rinds and seeds as well as fractions ranged from 8.72 ± 0,64 to 692.85 ± 41.11 mg/g sample. Whereas the total flavonoid levels in the samples ranged from 80.64 ± 6.98 to 680.15 ± 40.94 mg/g sample.