Hidrolisis Enzimatis Kertas Bekas Dengan Variasi Pemanasan Awal

Abstract

Enzymatic of cellulose hydrolysis is degradation process of polymer (cellulose )to be its monomer by using cellulase enzyme. Cellulose containing in the HVS paper is very high, almost 90 % of paper is cellulose Hhydrolysis of cellulose can be done by using acid, but this process has negative impact to the surrounding, so this research used enzymatic hydrolysis. The negative side of enzymatic hydrolysis is need very long time to degrade cellulose to be glucose. In this research, initial heating was done to decrease reaction time. Initial heating was done at 40oC for 30, 60, 120 and 240 minutes. The objective of initial heating is to increase the reaction rate without decreasing of enzyme activity as catalyze. The result show that initial heating for 30-60 minutes resulted the highest glucose, that was 481,1 – 482,4 mg of glucose from 5 gram of HVS paper.