Analisis Profil Metabolit Sekunder Ekstrak Lempuyang Emprit dengan Kromatografi Gas-Spekstroskopi Massa

Abstract

Lempuyang emprit (Zingiber amaricans Bl) is one of the plants species that contain secondary metabolites that are important in diseases treatment. This study was conducted to determine the secondary metabolites contained in the ethanol extract of lempuyang emprit from two regions (Semarang and Yogyakarta) after derivatized and determine its zerumbone level. Metabolite profile analysis performed by gas chromatography with mass spectroscopy detector, split injection system, and helium as the mobile phase at a constant rate of 3.0 mL/min and derivatized with BSTFA (N,O-bis-(trimetilsilil)-trifluoroasetamid). While zerumbone levels determined by the same method but without derivatization. The results showed that there were differences in secondary metabolite profiles of ethanol extract of lempuyang emprit from Semarang and Yogyakarta, and the zerumbone levels also differ in the two extracts were 24.04% w/w (Semarang) and 30.32% w/w (Yogyakarta).