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dc.contributor.authorHanwar, Dedi
dc.contributor.authorSuhendi, Andi
dc.contributor.authorTrisharyanti, Ika
dc.contributor.authorSantoso, Broto
dc.contributor.authorSafitri, Meisa
dc.contributor.authorHaryoto
dc.date.accessioned2015-02-03T06:29:07Z
dc.date.available2015-02-03T06:29:07Z
dc.date.issued2015-01-24
dc.identifier.citationAbdelwahab, S.I., Abdul A. B, Zain, Z. N., Abdul, A. H, 2012, Zerumbone inhibits interleukin-6 and induces apoptosis and cell cycle arrest in ovarian and cervical cancer cells, International Immunopharmacology, 12 (4) : 594-602 Backer, C. A., and Van Den Brink, R. C. B., 1965, Flora of Java: Spermatophytes only Volume 3, N. V. P. Noordhoff-GroningenThe Netherlands, 45 Ellis, D.I., Dunn, W.B., Griffin, J.L., Allwood, J.W., Goodacre, R., 2007, Metabolic Fingerprinting as A Diagnostic Tool, Pharmacogenomic Review, 8(9), 12431266 Desbrosses, G., Steinhauser, D., Kopka, J. & Udvardi, M., 2005. Metabolome Analysis Using GC-MS, Lotus Japonicus Handbook, Chapter 4.6, p. 166, Max-Planck-Institute Of Molecular Plant Physiology, Plant Nutrition Group, Germany Dunn, W.B., Bailey, N.J.C. & Johnson, H.E., 2005. Measuring the metabolome:current analytical technologies, Analyst, 130, 606625 Fancy, S.A., dan Rumpel, K., 2008. GC-MS Based Metabolomics, dalam Methods in Pharmacology and Toxicology: Biomarker Methods in Drug Discovery and Development, Humana Press, Totowa, hal 317–340 Fiehn, O., Kopka, J., Dormann, P., Altmann, T., Trethewey, R.N., Willmitzer, L., 2000, Metabolite profiling for plant functional genomics, Nat. Biotech. 18, 1157–1161 Harbone, 1988, Introduction Of Ecological Biochemistry. Edisi Ketiga. London. Academic Press Huang, G. C., Chien, T. Y. Chen, L. G., Wang, C. C., 2005, Antitumor effects of zerumbone from Zingiber zerumbet in P388D1 cells in vitro and in vivo, Plant Med. 71(3), 219-224 Keong Y.S., Alitheen, N. B., Mustafa, S., Aziz, S.A, Adul, R, M., Ali, A. M., 2010, Immunomodulatory Effects of Zerumbone Isolated from Roots of Zingiber zerumbet,Pak J Pharm Sci. 23(1) : 75-82 Kopka, J., 2006. Current Challenges and Developments in GC–MS Based Metabolite Profiling Technology, Journal of Biotechnology, 124 : 312–322 Mahdi H.J., Andayani R., Ishak, 2010, Metabolic Fingerprinting of Three Malaysian Ginger (Zingiber officinale Roscoe) Using Gas Chromatography-Mass Spectromertry, American Journal of Applied Sciences 7 (1): 17-23, 2010 Mukhlas, A., 2013, Penentuan Profil Kromatogram Ekstrak Etanol Lempuyang Emprit (Zingiber amaricans Bl.) dan Penetapan Kadar Zerumbon-Nya Dengan Metode Kromatografi Cair Kinerja Tinggi, Skripsi, Fakultas Farmasi, Universitas Muhammadiyah Surakarta Mulyani, S., 2010, Komponen dan antibakteri dari fraksi kristal minyak Zingiber zerumbet, Majalah Farmasi Indonesia, 21(3), 178 – 184 Mursyidi, A., 1989. Analisis Metabolite Sekunder. PAU Bioteknologi Universitas Gajah Mada, Yogyakarta, hal 1–7, 71–81 Oh, M.K., Rohlin, L., Kao K.C., Liao, J.C., 2002. Global expression profiling of acetategrown Escherichia coli. J. Biol. Chem., 277: 1375-1383 Riyanto, S., 2007, Identification Of Isolated Compounds From Zingiber amaricans Bl. Rhizome, Indo. J. Chem., 7 (1) : 93 – 96 Rohman, A., 2009, Kromatografi untuk Analisis Obat, Graha Ilmu, Yogyakarta, hal. 442-443 Saifudin, A., Rahayu, V., Teruna, H. Y., 2011, Standardisasi Bahan Obat Alam, Yogyakarta, Graha Ilmu Schauer, N., Steinhauser, D., Strelkov, S., Schomburg, D., Allison, G., Moritz, T.,et al., 2005, GC–MS libraries for the rapid identification of metabolites in complex biological samples, FEBS Letters, 579, 1332–1337 Somchit, M. N., 2012, Zerumbone Isolated from Zingiber zerumbet Inhibits Inflamation an Pain in Rats, Juornal of Medicinal Plants Research, 6 (2): 117-180 Sukari, M. A., Sharif, N. W. M., Yap, A. L. C., Tang, S. W., Noeh, B. K., Rahman M., et al., 2008, Chemical Constituents Variatiosns of Essential Oils from Rhizomes of Four Zingiberaceae Species, The Malaysian Journal of Analytical Sciences, 12 (3): 638644 Tjitrosoepomo dan Gembong, 1989, Taksonomi Tumbuhan obat-obatan, hal. 113, 130, 394, 421-423, hal. 113, 130, 394, 421423, Yogyakarta, UGM Press Villas-Boâs, S.G., Mas,S., Akesson, M., Smedsgaars, J. & Nielsen, J., 2005. Mass Spectrofotometry in Metabolome Analysis, Mass Spectrofotometry Review, 24, 613-646 Want, E. J., Cravatt, B. F., Siuzdak, G., 2005, The Expanding Role of Mass Spectrometry in Metabolite Profiling and Characterization, ChemBioChem, 6, 1941-1951.en_US
dc.identifier.issn2407-9189
dc.identifier.urihttp://hdl.handle.net/11617/5161
dc.description.abstractLempuyang emprit (Zingiber amaricans Bl) is one of the plants species that contain secondary metabolites that are important in diseases treatment. This study was conducted to determine the secondary metabolites contained in the ethanol extract of lempuyang emprit from two regions (Semarang and Yogyakarta) after derivatized and determine its zerumbone level. Metabolite profile analysis performed by gas chromatography with mass spectroscopy detector, split injection system, and helium as the mobile phase at a constant rate of 3.0 mL/min and derivatized with BSTFA (N,O-bis-(trimetilsilil)-trifluoroasetamid). While zerumbone levels determined by the same method but without derivatization. The results showed that there were differences in secondary metabolite profiles of ethanol extract of lempuyang emprit from Semarang and Yogyakarta, and the zerumbone levels also differ in the two extracts were 24.04% w/w (Semarang) and 30.32% w/w (Yogyakarta).en_US
dc.publisherUniversitas Muhammadiyah Surakartaen_US
dc.subjectZingiber amaricans Blen_US
dc.subjectGC-MSen_US
dc.subjectBSTFAen_US
dc.subjectMetabolite profiling,en_US
dc.subjectzerumboneen_US
dc.titleAnalisis Profil Metabolit Sekunder Ekstrak Lempuyang Emprit dengan Kromatografi Gas-Spekstroskopi Massaen_US
dc.typeArticleen_US


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