Identifikasi Bakteri Batang Gram Negatif Pada Darah Widal Positif Berdasarkan Karakter Fenotipik
Artama, Wayan T.
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Sensitivity, specificity, and predictive value of Widal test were varied followed by the success values of blood cultures by 40%-89%. The purpose of this study was to identify Gram-negative bacillus bacteria in positive Widal blood cultures based on phenotypic. One hundred and thirty six (136) samples were gathered from in and outpatients of 4 hospitals and 2 community health centers in Semarang. Bact/Alert FAN, Mac Conkey, Rapid Test Kit API 20E, API 50CHB/E were used Blood culture, isolation and characterizations. Sensitivity test to 6 types of antibiotics were used for phenotypic characterization. The results were classified into four clusters. Cluster I consisted of 5 isolates of Salmonella typhi (KD 30.4, SA 02.2, KD 30.3, NCTC 786, BA 07.4) with similarity value of 91.8%-97.4%. Cluster II: Escherichia coli (BA 30.1 ; BA 30.2) and Salmonella sp. BA 30.5 (88.2%-97.4%). Cluster III, Serratia marcescens KD 08.4 and KD 08.5 (94.7%). Cluster IV, Enterobacter cloacae BA 45.4.1, TG 03.5, KT 16, SA 02.1 and 1 isolate of Klebsiella pneumoniae KD 58.4 (83.9%-94.7%) . The results of sensitivity test of 14 bacterial isolates and one reference strain of S. typhi NCTC 786 showed that 93.3 % was sensitive to chloramphenicol, 86.7 % to ciprofloxacin, 66.7 % to gentamicin, respectively to cefotaxime (60%), trimethoprim-sulfametoksasol (60%), and ampicillin (53.3%). Bacterial species diversity in blood caused sensitivity and specificity of Widal various. The bacterial sensitivity to chloramphenicol was still high.