Identifikasi Bakteri Batang Gram Negatif Pada Darah Widal Positif Berdasarkan Karakter Fenotipik

Abstract

Sensitivity, specificity, and predictive value of Widal test were varied followed by the success values of blood cultures by 40%-89%. The purpose of this study was to identify Gram-negative bacillus bacteria in positive Widal blood cultures based on phenotypic. One hundred and thirty six (136) samples were gathered from in and outpatients of 4 hospitals and 2 community health centers in Semarang. Bact/Alert FAN, Mac Conkey, Rapid Test Kit API 20E, API 50CHB/E were used Blood culture, isolation and characterizations. Sensitivity test to 6 types of antibiotics were used for phenotypic characterization. The results were classified into four clusters. Cluster I consisted of 5 isolates of Salmonella typhi (KD 30.4, SA 02.2, KD 30.3, NCTC 786, BA 07.4) with similarity value of 91.8%-97.4%. Cluster II: Escherichia coli (BA 30.1 ; BA 30.2) and Salmonella sp. BA 30.5 (88.2%-97.4%). Cluster III, Serratia marcescens KD 08.4 and KD 08.5 (94.7%). Cluster IV, Enterobacter cloacae BA 45.4.1, TG 03.5, KT 16, SA 02.1 and 1 isolate of Klebsiella pneumoniae KD 58.4 (83.9%-94.7%) . The results of sensitivity test of 14 bacterial isolates and one reference strain of S. typhi NCTC 786 showed that 93.3 % was sensitive to chloramphenicol, 86.7 % to ciprofloxacin, 66.7 % to gentamicin, respectively to cefotaxime (60%), trimethoprim-sulfametoksasol (60%), and ampicillin (53.3%). Bacterial species diversity in blood caused sensitivity and specificity of Widal various. The bacterial sensitivity to chloramphenicol was still high.