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dc.contributor.authorDarmawati, Sri
dc.contributor.authorSembiring, Langkah
dc.contributor.authorAsmara, Widya
dc.contributor.authorArtama, Wayan T.
dc.date.accessioned2015-02-03T07:20:57Z
dc.date.available2015-02-03T07:20:57Z
dc.date.issued2015-01-24
dc.identifier.citationAmarantini, C., W. Asmara, H. Kushadiwijaya & L. Sembiring. 2009. Seleksi Bakteri Salmonella typhi dari Kultur Darah Penderita Demam Tifoid. Prosiding Seminar Nasional Penelitian, Pendidikan dan Penerapan MIPA. Fakultas MIPA. Universitas Negeri Yogyakarta. ISBN: 978-979-96880 Anonim. 2008. Profil Kesehatan Kota Semarang 2008. DinasKesehatan. Jl. Pandanaran 79 Semarang Crump, J.A., F.G. Youssef, S.P. Luby, M.O. Wasfy, J.M. Rangel, M. Taalat, S.A. Oun and F.J.Mahomey, 2004. Estimating the Incidence of Typhoid Fever and othe Febrile Illness in Developing Countries. Emerging Infectious Diseases. Vo. 9, No. 5: 539544 McDonald, L.C., J. Fune, L. B. Gaido, M.P. Weinstein, L.G. Reimer, T.M. Flynn, M.L. Wilson, S. Mirrett & L.B. Reller. 1996. Clinical Importance of Increased Sensitivity of BacT/Alert FAN Aerobic and Anaerobic Blood Culture Bottles. Journal of Clinical Microbiology. 34 (9): 2180-2184 Moehario, L.H. 2009. The molecular epidemiology of Salmonella Typhi across Indonesia reveals bacterial migration. Department of Microbiology, Faculty of Medicine, University of Indonesia, Jalan Pegangsaan Timur 16, Jakarta J Infect Dev Ctries 2009; 3(8):579-584. Novianti, T. 2006. Pemeriksaan Anti Salmonella typhi IgM Untuk Diagnosis Demam Tifoid. Informasi Laboratorium. ISSN 0854-7165. No. Olsen, S.J., J. Pruckler, W. Bibb, N.T.M. Tanh, T.M. Trinh, N.T. Minh, S. Silvapalasingam, A. Gupta, P.T. Phuong, N.T. Chinh, N.V. Chau, P.D. Cam and E.D. Mintz, 2004. Evaluation of Rapid Diagnostic test for Typhoid Fever. J. Clin. Microbiol. Vol. 42, No. %: 1885-1889 Punjabi, N.H. 2004. Demam Tifoid dan Imunisasi Terhadap Penyakit ini. U.S. NAMRU-2, Jakarta. http://www.papdi. Or.id/Imunisasi/demam typhoid dan imunisasi terh.htm Sembiring, L. 2004. Peranan Biosistematika Dalam Menunjang Pemanfaatan Keanekaragaman Hayati. Seminar Nasional Biologi. 25 September 2004. Institut Teknologi Sepuluh Nopember. Surabaya Suharjono, Sembiring, L., Subagja, J., Ardyati, T. dan Lisdiana, L. 2007. Sistematik Numerik Strain-strain Anggota Genus Pseudomonas Pendegradasi Alkilbenzen Sulfonat Liniar Berdasarkan Sifat Fenotip dan Protein Fingerprinting. Biota, 12 (1): 47−54 Thong, KL., Altwegg, M., Pang, T. 2000. Comparative Analysis of Salmonella typhi by rRNA Gene Restriction and Phage Typhing. Pakistan Journal of Biological Sciences. 3 (5): 738-739 Vollaarrd, AM., Soegianto, A., Suwandhi, W., Henri A.G.H. van Asten, Leo G. V. Charles, S., J.T. van Dissel, 2005. Identification of typhoid fever and paratyphoid fever at presentation in outpatient clinics in Jakarta, Indonesia. Royal Society of Tropical Medicine and Hygiene. Elsever. Vol. 99: 440450 WHO, 2003. Background Document: The Diagnosis, Treatment and Prevention of Typhoid Fever. Communicable Disease Surveillance and Response Vaccines and Biologicals.en_US
dc.identifier.issn2407-9189
dc.identifier.urihttp://hdl.handle.net/11617/5169
dc.description.abstractSensitivity, specificity, and predictive value of Widal test were varied followed by the success values of blood cultures by 40%-89%. The purpose of this study was to identify Gram-negative bacillus bacteria in positive Widal blood cultures based on phenotypic. One hundred and thirty six (136) samples were gathered from in and outpatients of 4 hospitals and 2 community health centers in Semarang. Bact/Alert FAN, Mac Conkey, Rapid Test Kit API 20E, API 50CHB/E were used Blood culture, isolation and characterizations. Sensitivity test to 6 types of antibiotics were used for phenotypic characterization. The results were classified into four clusters. Cluster I consisted of 5 isolates of Salmonella typhi (KD 30.4, SA 02.2, KD 30.3, NCTC 786, BA 07.4) with similarity value of 91.8%-97.4%. Cluster II: Escherichia coli (BA 30.1 ; BA 30.2) and Salmonella sp. BA 30.5 (88.2%-97.4%). Cluster III, Serratia marcescens KD 08.4 and KD 08.5 (94.7%). Cluster IV, Enterobacter cloacae BA 45.4.1, TG 03.5, KT 16, SA 02.1 and 1 isolate of Klebsiella pneumoniae KD 58.4 (83.9%-94.7%) . The results of sensitivity test of 14 bacterial isolates and one reference strain of S. typhi NCTC 786 showed that 93.3 % was sensitive to chloramphenicol, 86.7 % to ciprofloxacin, 66.7 % to gentamicin, respectively to cefotaxime (60%), trimethoprim-sulfametoksasol (60%), and ampicillin (53.3%). Bacterial species diversity in blood caused sensitivity and specificity of Widal various. The bacterial sensitivity to chloramphenicol was still high.en_US
dc.publisherUniversitas Muhammadiyah Surakartaen_US
dc.subjectWidalen_US
dc.subjectKultur darahen_US
dc.subjectBacT/Alert FANen_US
dc.subjectAPI 20Een_US
dc.subjectAPI 50 CHB/Een_US
dc.titleIdentifikasi Bakteri Batang Gram Negatif Pada Darah Widal Positif Berdasarkan Karakter Fenotipiken_US
dc.typeArticleen_US


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