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| dc.description.abstract | Sensitivity, specificity, and predictive value of Widal test were varied followed by the success
values of blood cultures by 40%-89%. The purpose of this study was to identify Gram-negative
bacillus bacteria in positive Widal blood cultures based on phenotypic. One hundred and thirty
six (136) samples were gathered from in and outpatients of 4 hospitals and 2 community health
centers in Semarang. Bact/Alert FAN, Mac Conkey, Rapid Test Kit API 20E, API 50CHB/E
were used Blood culture, isolation and characterizations. Sensitivity test to 6 types of antibiotics
were used for phenotypic characterization. The results were classified into four clusters. Cluster
I consisted of 5 isolates of Salmonella typhi (KD 30.4, SA 02.2, KD 30.3, NCTC 786, BA 07.4)
with similarity value of 91.8%-97.4%. Cluster II: Escherichia coli (BA 30.1 ; BA 30.2) and
Salmonella sp. BA 30.5 (88.2%-97.4%). Cluster III, Serratia marcescens KD 08.4 and KD 08.5
(94.7%). Cluster IV, Enterobacter cloacae BA 45.4.1, TG 03.5, KT 16, SA 02.1 and 1 isolate of
Klebsiella pneumoniae KD 58.4 (83.9%-94.7%) . The results of sensitivity test of 14 bacterial
isolates and one reference strain of S. typhi NCTC 786 showed that 93.3 % was sensitive to
chloramphenicol, 86.7 % to ciprofloxacin, 66.7 % to gentamicin, respectively to cefotaxime
(60%), trimethoprim-sulfametoksasol (60%), and ampicillin (53.3%).
Bacterial species diversity in blood caused sensitivity and specificity of Widal various. The
bacterial sensitivity to chloramphenicol was still high. | en_US |
