Effect Of Temperature and Storage Duration On Lactose, Protein And Fat Content Of Breast Milk
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Date
2016-05-27Author
Iqbal, Muhammad
Lestari, Lily A.
Kurdanti, Weny
Mardiyati, Nur Lathifah
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Show full item recordAbstract
Many working mothers stop breastfeeding their babies while actually breast milk can be
stored and fed to babies while mothers are working, as long as the storing process is carried
out properly. Thus, it is important to understand the most appropriate way to store breast milk.
The objective of the study was to assess the influence of different temperatures and storing
durations on the contents of lactose, protein and fat in breast milk. This research was an
experimental study conducted in the laboratory with a complete randomized design. Mature
breast milk samples were obtained from 21 volunteer mothers, at least two weeks after they
delivered their babies. Samples were divided into three treatment-groups of storing
temperatures: room, refrigerator and freezer temperatures. The measurement of the contents
of lactose, protein and fat performed in each sample on the 2
nd
and 3
rd
day. Statistical analysis
was carried out by Anova with the degree of significance of 99% and followed with Duncan
tests. There were insignificant differences of storage temperatures (P>0.01) in the lactose,
protein and fat contents of breast milk, with p-value of 0.893, 0.973, 0.950, respectively.
Furthermore, there was insignificant impact of the storing duration on lactose content of
breast milk with p-value of 0.096. On the contrary, the storing duration affected the protein
and fat contents of breast milk significantly, each showed p-value: 0.001. The temperature of
storage did not affect the contents of lactose, protein and fat of breast milk. Meanwhile, the
storage duration influenced the protein and fat content. Based on this research, breast milk can
be stored up to 72 hours in the refrigerator or freezer temperature to keep the nutritien
contents intact.