Uji Aktivitas Ekstrak Daun Pandan Wangi (Pandanus Amaryllifolius Roxb.) sebagai Antibakteri

Abstract

Pandanus amaryllifolius Roxb. leaves k nowen weel as antibacterial agent. The aim of this research was to k now the activity of ethyl acetate extract of Pandanus amaryllifolius Roxb. leaves toward ba cteria isolates which was isolated from dan druff. this research was ek sploration research. The steps in this rese arch include: 1). Isolation bacteria from dan druff, 2). Purif ication the bacteria isolates, 3). Made the ethyl a cetate extract of Pandanus amaryllifolius Roxb. leaves, 4 ). Inhibition test of the extract to bacteria isolates, and 5). Identification of the active compo und in the Pandanus amaryllifolius Roxb. leaves extract. based on the research, it was k nowen that the density of bacteria in the dand ruff was 3,4 x 107 colony/gram. There were 28 pu re isolates ba cteria from the pu rification process. The inhibition test showed that Pandanus amaryllifolius Roxb. leaves extract could inhibit 92,86% bacteria growth with diameter of inhibition zo ne about 13 to 40 mm. Beside that, it was knowen that 8 isolates bacteria cou ld be inhibited by the extract with ston g category, namely: isolate B4a (diameter inhibition zone 40 mm), B4b (30 mm), B4c (27 mm), B6a (35 mm), B10a (25 mm), B14a (34 mm), B16b (27 mm), an d B16c (35 mm). The active substance in Pandanus amaryllifolius Roxb. leaves extract were : ph enol (9,42% b/b) and flavonoid (4,39% b/b). Based on this research, it can be conclused that Pandanus amaryllifolius Roxb. leaves ha ve a potency to be mad e anti dandruff shampoo.